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(A) Eu-H3/INSL5 binding to mutant receptors expressed on the surface of cells. Data are expressed as a ratio of specific Eu-H3/I5 binding to the cell surface expression (B) of each construct and are normalized to HA-RXFP3 binding. (B) Cell surface expression of the various RXFP3 mutant receptors compared to HA-RXFP3. All data are the mean of triplicate determination from 4 to 8 independent experiments. *p < 0.001 vs HA-RXFP3, #p < 0.01 vs HA-RXFP3.
