Fig. 1.
In vitro and in vivo characterization of luciferase gene-transduced hGPs. (A) In vitro BLI of transduced hGP shows an increasing luciferase activity with increasing cell number. (B) Data were obtained in triplicate, showing a linear correlation between BLI signal and cell concentration (R2 = 0.99). Each point represents the mean value ± SEM. (C) In vivo BL images following transplantation of transduced hGPs show signal only for transduced cells and luciferin administration. (D and E) An in vivo cell dilution curve was obtained allowing the correlation of BLI signal to the total cell number, with the BLI signal decreasing at day 1 after transplantation. Each point represents the mean value ± SEM. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]