Figure 5. BAX modulates leukemia cell sensitivity to ABT-737.
Transduced REH or MOLM-13 cells [virus encoding either negative control shRNA (shC) or BAX-specific shRNA (shBAX)] were incubated with ABT-737 for 24 hours, and the Annexin V-positive fractions were measured by flow cytometry. Results are expressed as mean ± SD. Intensity of the immunoblot signals was determined by densitometry using NIH ImageJ 1.44 software, and the intensity of BAX relative to that of β-actin was calculated. *P < .01.