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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Biochemistry. 2013 Feb 5;52(7):1227–1235. doi: 10.1021/bi301646n

Figure 5.

Figure 5

Stability of the A•B´ and A´•B complexes. (A) E´-catalyzed ligation of A and B in the presence of no B´ (black), 0.1 µM B´ (gray), or 1 µM B´ (white); or (B) E-catalyzed ligation of A´ and B´ in the presence of no B (black), 0.1 µM B (gray), or 1 µM B (white); employing various concentrations of enzyme. The data were fit to Eq. 3 (see Experimental Procedures) to obtain the Kd of the A•B´ and A´•B complexes.