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Accumulation of OspA in transplastomic rootless biomass and its quantification. Total soluble protein was extracted from OA14 (a) and OA:YFP (b) grown as a cell-suspension culture (Cells) or in a TIB and subjected to SDS-PAGE followed by immunoblotting. Samples from WT (Cells Wt and TIB Wt) were used as controls. Different amounts of purified OspA from E. coli were used as standards
