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Proof of the absence of bacteria in Nepenthes pitcher fluids. PCR-based amplification of highly conserved 16S-rDNA sequence (200 bp) using selected domain-specific primers. Lanes: 1, N. alata; 2, N. fusca; 3, N. mirabilis; 4, N. superba; 5, N. thorelii, 6, N. ventricosa; M: 100-bp DNA ladder; NC, negative control without cells; PC, positive control using E. coli cells.
