Skip to main content
. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 13;189(6):2735–2745. doi: 10.4049/jimmunol.1102038

Figure 1. pDC produce IFN-λ in response to viruses and CpGA.

Figure 1

A) Induction of IFN- λ1 and IFN- λ2 mRNA in PBMC and purified pDC. Cells were treated with HSV (MOI=1) for 7 hr. The mRNA levels of IFN-λ1 (i) and IFN-λ2 (ii) were determined by real-time RT-PCR. Data represent mean ± 1 SD (n=3 separate experiments). B) Virus-induced production of IFN-λ1 by pDC in PBMC suspensions. pDC were distinguished from other PBMC cell types by simultaneous detection of BDCA-2 and CD123 markers (i) gating for pDC; PBMC were stimulated with (ii) HSV, Flu, or SeV or, (iii) AT-2 inactivated HIVADA or HIVMN for 7 hr, then stained for intracellular IFN-λ and intracellular expression of IFN-λ1/3 was detected by flow cytometry in gated pDC populations. Data are representative of at least 3 separate experiments. C) HSV-induced IFN-λl production by purified pDC. (i) pDC were negatively enriched using magnetic bead sorting and (ii) expression of intracellular IFN-λ1 in the purified pDC was detected by flow cytometry following 7 hr stimulation with HSV-1. Data are representative of 3 experiments.