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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 13;189(6):2735–2745. doi: 10.4049/jimmunol.1102038

Figure 8. IFN-λ production by pDC is sensitive to treatment with dexamethasone (Dex) and exogenous IFN-λ partially protects from Dex-mediated apoptosis.

Figure 8

A) Enriched pDC were treated with 1 µM dexamethasone (Dex) for 1 hr prior to stimulation with HSV-1, HIV-1 or highly purified GFP-HSV for another 18 hr. IFN-λ in supernatants was determined by ELISA. Data represent mean and range for two separate donors. B) Exogenous IFN-λ inhibits apoptosis of pDC. PBMC were treated with Dex, with or without IFN-α (10,000 U/ml) or IFN-λ1 (10ng/ml) for 6 hr. Active caspase-3 in pDC was stained intracellularly in pDC. Data are representative of 3 different donors. C) Annexin V/PI staining was carried out for pDC within PBMC treated with 1 µM Dex in the presence or absence of rIFN-α (100 ng/ml) or –λ1 (10 or 100 ng/ml). Data are representative of three experiments.