Skip to main content
. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2013 Mar;72(3):219–233. doi: 10.1097/NEN.0b013e3182859939

Figure 3.

Figure 3

Epidermal growth factor (EGF), like neuregulin-1β (NRG1β), optimally stimulates malignant peripheral nerve sheath tumor (MPNST) cell migration assays on a laminin substrate. (A) Human ST88-14 MPNST cells were plated on the upper surface of Transwell filter inserts coated with collagen type I, fibronectin or poly-L-lysine/laminin and allowed to migrate for 6 hours. Vehicle, 0.1 nM NRG1β (a concentration we have previously determined optimally promotes the migration of this line), or 10 nM EGF was added to the bottom chamber. The bar graph indicates the average number of ST88-14 MPNST cells migrating to the undersurface of filters coated with the indicated substrates. *p < 0.05 vs. cells receiving vehicle and migrating on the same substrate. (B) ST88-14 cells were plated on the upper surface of Transwell filter inserts coated with poly-L-lysine alone or poly-L-lysine/laminin and allowed to migrate for 6 hours. Migration on poly-L-lysine/laminin is considered 100%, with migration on poly-L-lysine normalized to this value. *p < 0.05 for the comparison of these 2 conditions. (C) Bar graphs indicating the relative migration of 4 MPNST cell lines challenged with vehicle or 0.1 to 100 nM concentrations of EGF. SEM are indicated for each bar. *p < 0.05 vs. the migration of the same cell lines receiving vehicle rather than EGF.