Table 2.
Prevalence of E. coli and Shigella specific genes detected by PCR and secreted StcE antigen and C1 cleavage activity detected by immunoblot.
| Straina | Species | E. coli O157:H7 | S. flexneri 5a | StcE secretion | StcE activity | ||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| plasmid
|
chromosome
|
plasmid
|
|||||||
| stcE/etpD | katP | ler/espA | cadA | ipaB/ipaD/icsA/mxiM | virK/ipaH7.8 | ||||
| EDL933 | E. coli O157:H7 | + | + | + | + | − | − | + | + |
| M90T | S. flexneri 5a | − | − | − | − | + | + | − | − |
| 5216–70 | Atypical S. boydii 13 | − | − | − | − | − | + | − | − |
| 3556–77 | Atypical S. boydii 13 | + | − | + | − | − | − | + | + |
| 3557–77 | Atypical S. boydii 13 | + | − | + | − | − | − | − | − |
| 3052–94 | Atypical S. boydii 13 | + | − | + | − | − | − | + | + |
| 3053–94 | Atypical S. boydii 13 | + | − | + | − | − | − | + | + |
a
stcE was not detected in the following additional strains tested: S. boydii 13 3103–99, S. boydii 13 ATCC
12032, S. boydii 13 2045–54, S. boydii 13 K-694, S. boydii 7 K-1, E. albertii 9194, E. albertii 10790,
E. albertii 10457, E. albertii 12502, E. albertii 19982.