Skip to main content
. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: Mol Cell Proteomics. 2006 Dec 27;6(3):413–424. doi: 10.1074/mcp.M600437-MCP200

Fig. 5. Schematics of ORF transfer by recombinational cloning to generate expression clones for subcellular localization studies.

Fig. 5

ORF-T form contains the stop codon, whereas ORF-N form lacks the stop codon. ORF sequence is transferred to expression vectors via LR recombination. The T form is CFP- or YFP-tagged at the N terminus; the N form is CFP- or YFP-tagged at the C terminus.