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. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: Science. 2006 Sep 21;314(5804):1454–1457. doi: 10.1126/science.1131163

Fig. 4.

Fig. 4

Translocation of PI 3-kinase without change of KCNQ current amplitude. (A) The translocatable inter-SH2 domain of p85 and its complexation with endogenous p110 PI 3-kinase. PtdIns(3,4,5)P3, PI(3,4,5)P3. (B) Translocation of CF-iSH and YFP-PH(Akt) in NIH3T3 cells after a 1-min addition of iRap (5 µM). (C) Time course of translocation of CF-iSH and YFP-PH(Akt) by iRap. Error bars indicate SEM. (D) Translocation in cells coexpressing CF-iSH and YFP-PH(PLC-δ). (E) Modulation of KCNQ2/KCNQ3 channel activity by iRap in a tsA cell expressing LDR plus CF-iSH in serum-free conditions. Inset shows the current change after 4 min of iRap; mean, −0.4 ± 2.0% (n = 5 cells).