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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Microcirculation. 2012 Oct;19(7):610–618. doi: 10.1111/j.1549-8719.2012.00194.x

Figure 1.

Figure 1

A. Schematic of the surgical procedure and the three regions of the gracilis artery. FA: femoral artery. MB: muscular branch of FA. SA: saphenous artery. GA: gracilis artery. B. Micrograph of a paraffin section stained for smooth muscle and cell nuclei by immunohistochemistry using fluorescently labeled anti smooth muscle α-actin (red/yellow) antibody and bisbenzimidazole (BBI, blue), respectively. The medial cross-section area was estimated from measurements of the α-actin positive area. Endothelial cell nuclei are represented by the BBI positive structures inside the vessel lumen (arrows), while the smooth muscle cell nuclei are the BBI structures overlapping the α-actin positive areas (stars). GA: gracilis artery, GV: gracilis vein, GM: gracilis muscle fibers.