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Experimental measurements of fluorescence resonance energy transfer. (A) Cartoon illustrating the principle of FRET: the amount of energy transfer depends on the distance between an excited donor fluorophore (green star) and acceptor fluorophore (red star). (B) Basic set-up of a prism-based single-molecule FRET microscope. (C) Basic set-up of a zero-mode waveguide (ZMW)-based fluorescent microscope. (88, 96, 97, 100)
