Table 1.
Adducted sequences on actin observed with metabolites of interest.
| Actin Observed Adducted Peptides by Reactive Metabolites of Naphthalene | |||||
|---|---|---|---|---|---|
| Peptide Order |
Sequence | Predicted Adducted Mass |
Charge | Observed Mass |
Modifier |
| 1 – 18 | MEEEIAALVIDNGSGMCK | 2053.9304 | 2 | 1026.9643 | NPO |
| 1 – 18 | MEEEIAALVIDNGSGMCK* | 2087.9332 | 2 | 1043.9679 | NDO |
| 1 – 18 | MEEEIAALVIDNGSGMCK | 2067.9070 | 2 | 1033.9498 | 1,2NPQ |
| 1 – 18 | MEEEIAALVIDNGSGMCK | 2067.9070 | 2 | 1033.9521 | 1,4NPQ |
| 40 – 50 | HQGVMVGMGQK | 1349.6240 | 2 | 674.8164 | NDO |
| 40 – 50 | HQGVMVGMGQK | 1329.5978 | 2 | 664.7988 | 1,2NPQ |
| 40 – 50 | HQGVMVGMGQK | 1329.5978 | 2 | 664.7953 | 1,4NPQ |
| 63 – 68 | GILTLK | 802.4608 | 1 | 802.4601 | 1,4NPQ |
| 85 – 95 | IWHHTFYNELR | 1673.7758 | 2 | 836.8874 | 1,2NPQ |
| 96 – 113 | VAPEEHPVLLTEAPLNPK | 2132.1170 | 2 | 1066.0590 | NDO |
| 182 – 196 | DLTDYLMKILTER* | 1754.8935 | 2 | 877.4465 | NPO |
| 210 – 214 | DIKEK | 790.3878 | 1 | 790.3892 | 1,4NPQ |
| 257 – 283 | CPEALFQPSFLGMESCGIHETTFNSIMK* | 3275.4458 | 3 | 1091.8143 | 1,2NPQ |
| 328 – 335 | IKIIAPPER* | 1180.6985 | 2 | 590.3488 | NPO |
The diadduct is highlighted in gray.
Binding sites on the peptides are underlined and in bold.
*
represents observed adducted sequences that did not have strong MS/MS fragmentation patterns.
Spectra are shown in supplemental data.