Figure 4.

Genetically encoded calcium imaging of olfactory bulb activity during development. (A) Inverse pericam expression driven by the neuronal promoter HuC labels cells in the olfactory bulb (left). Color-coded response patterns from the area outlined in green captured using wide-field epifluorescence and a cooled CCD camera are to the right and below. The responses to 14 different odor stimuli at 3 and 5 days post fertilization (dpf) from the same larva are illustrated. While signals are certainly stronger in older larvae, the broad spatial organization of odor responses is already apparent early on. Image reproduced from Li et al. (2005) with permission (Copyright 2005 by the Society for Neuroscience). Odorant abbreviations can be found in the original citation.