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. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4609–4617. doi: 10.4049/jimmunol.1002008

FIGURE 2.

FIGURE 2

Transgenic Lfng competitive advantage is restricted to DN3b thymocytes undergoing β-selection. Equal numbers of Lin− bone marrow progenitors from Lfng Tg+ B6.CD45.2 and WT B6.CD45.1;CD45.2 mice were intrathymically injected into WT B6.CD45.1 hosts. Abs against Lineage markers (CD4, CD8, CD3ε, B220, Mac-1, GR-1, and NK1.1), CD25, CD44, CD27, CD45.1, and CD45.2 were used to assess the relative contribution of Lfng Tg+ and WT cells to the DN3a and DN3b subsets of individual thymic lobes 18 d later. A, Gating strategy used to assess donor chimerism in the DN3a and DN3b thymocyte pools. B, Relative contribution of Lfng Tg+ and WT donor cells to DN3a and DN3b thymocytes subsets in Lfng Tg+ + WT chimeras and control WT + WT chimeras. Each experiment was performed at least three times with similar results.