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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Circ Res. 2012 Nov 19;112(2):289–297. doi: 10.1161/CIRCRESAHA.111.300003

Figure 6. Increased levels of PKA and CaMKII dependent phosphorylation of SR Ca2+ release channels in PDE3A−/− hearts.

Figure 6

A) Western blots of WT and PDE3−/− heart lysates (50 μg/lane), illustrating the effect of PDE3A ablation on phosphorylation of SR Ca2+ release channel (RyR2) at PKA-dependent sites Serine 2808(pS2808, Abcam 59225), Serine 2030 (pS230, Badrilla A010-32) and CaMKII dependent site serine 2814 (pS2814, Badrilla A010-31). Calreticulin was used to confirm equal loading between the samples. B) Bar graph summarizing pRyR2/RyR2 ratios in WT and PDE3A−/− hearts. ~2 fold increase in PDE3−/− lysates at all three sites.*p< 0.01 versus WT (n=2 independent experiments, each with duplicate samples from 3 WT and 3 PDE3−/− heart lysates).