Table 2.

Calibration curve statistics for YRPVDQY and RPVDQY MRM quantification transitions.^a

YRPV*DQY, b6 transition
ExpectedAmount (fmol)	# of replicates	Calculated Amount (fmol)	Standard Deviation	RSD	Accuracy
0.080	5	0.080	0.002	2.0	99.5
0.14	5	0.14	0.007	4.9	97.5
0.40	5	0.42	0.01	3.4	104.7
8.0	5	8.4	0.3	3.3	105.4
40	5	35	2	4.6	86.6
100	5	108	2	1.9	107.8
200	5	197	10	5.2	98.5
RPV*DQY, b5 transition
ExpectedAmount (fmol)	# of replicates	Calculated Amount (fmol)	Standard Deviation	RSD	Accuracy
0.080	5	0.069	0.007	10.6	86.5
0.14	5	0.15	0.02	11.1	107.6
0.40	5	0.40	0.04	10.3	99.7
40	5	45	5	10.3	111.7
100	5	94	12	13.0	93.5
200	5	202	24	11.6	100.9

^aThe RSD and accuracy of each point on the calibration curve are < 15% of the real value.