Table 3.

Assay performance

Organisma	Target	Efficiency (%)	Linear range (fg/reaction)	Repeatability (SD of Cq)b	LOD gDNA (fg/reaction)c
B. mallei	BuMC	99,0	1.4·10-1 – 1.4·106	0,072	0.2
	mau	98,7	1.4·100 – 1.4·106	0,080	4.5
B. pseudomallei	BuMC	96,6	1·100 - 1·107	0,085	3,7
	psu	99,2	1·101 – 1·107	0,077	57

^a Organism from which genomic DNA was used for assessment of assay performance.

^b Values represent the average from the standard deviations calculated at 6 different dilutions from 4 replicate Cq measurements.

^c Values displayed represent the lowest DNA concentration at which 95% of the positive samples are detected, as calculated by using probit analysis.