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IFN-γ ELISPOT results from gp100 AuNV induction of pmel-1 splenocytes. At 1011 particles/ml or 25 μg/ml, AuNVs stimulated threefold more IFN-γ secreting cells compared to the free-peptide control. At 1010 particles/ml or 2.5 μg/ml maximum dose, the gp100 AuNVs exhibited similar effects as the free-peptide control (10 μg/ml) with no significant difference (p = 0.4).
