Table 1.
Primers used for the full-length cloning and expression of the porcine MAP1LC3A gene
| Primer name | Sequence | Size |
|---|---|---|
|
cDNA cloning primers | ||
|
GeneRacer 3′-primer |
5′GCTGTCAACGATACGCTACGTAACG 3′ |
|
|
GeneRacer 3′-nested primer |
5′CGCTACGTAACGGCATGACAGTG 3′ |
|
|
GeneRacer 5′-primer |
5′CGACTGGAGCACGAGGACACTGA 3′ |
|
|
GeneRacer 5′-nested primer |
5′GGACACTGACATGGACTGAAGGAGTA 3′ |
|
|
5′RACE-Rv primer 1 |
5′TGTCCAGGACTGGCAGCTGCTTCT 3′ |
|
|
5′RACE-Rv primer 2 |
5′CCAACTCGCTCATGTTGACATGGT 3′ |
|
|
3′RACE-Fw primer |
5′ATCGAGCGCTACAAGGGTGAGAAGC 3′ |
|
|
Gene expression primers | ||
|
Porcine GAPDH Fw |
5′CCCGTTCGACAGACAGCCGTG 3′ |
238 |
|
Porcine GAPDH Rv |
5′CCGCCTTGACTGTGCCGTGG 3′ |
|
|
Porcine MAP1LC3A Fw |
5′AGAAGCAGCTGCCAGTCCTGGACA 3′ |
687 |
|
Porcine MAP1LC3A Fw |
5′CAGGCAGGCCTGAGCAATCTTTATT 3′ |
|
|
Porcine ATG5 Fw |
5′AGAGAAGTCTGTCCTTCCGCAGTCG 3′ |
241 |
| Porcine ATG5 Rv | 5′AAGCAGAAGGGTGACATGCTCTGGT 3′ | |