Table 4.
Primers used for PCRmethods
| Primer name | Sequence 5′-3′ | Application | Size | Number of CpGs | Ref |
|---|---|---|---|---|---|
| MLH1-C-FW |
GTATTTTTGTTTTTATTGGTTGGATA |
COBRA |
294 bp |
1 |
[24] |
| MLH1-C-RE |
AATACCTTCAACCAATCACCTCAATA |
COBRA |
|
|
|
| MLH1-1-FW |
GGAGAGGAGGAGTTTGAGAAG |
PSQ |
269 bp |
7 |
|
| MLH1-1-RE |
Biotin-AATACCAATCAAATTTCTCAACTCTAT |
PSQ |
|
|
|
| MLH1-1-Seq |
TTGTTTTTATTGGTTGGATAT |
PSQ |
|
|
|
| MGMT-FW |
Biotin-GGATATGTTGGGATAGTT |
PSQ |
266 bp |
5 |
[42] |
| MGMT-RE |
AAACTAAACAACACCTAAA |
PSQ |
|
|
|
| MGMT-Seq |
CCCAAACACTCACCAAA |
PSQ |
|
|
|
| MLH1-Q-FW |
TTCTCAGGTTATCGGAGCCAGCAC |
qRT-PCR |
288 bp |
|
[43] |
| MLH1-Q-RE |
CTTCGTCCCAATTCACCTCAGTGG |
qRT-PCR |
|||
| DNMT1-Q-FW |
ACCGCTTCTACTTCCTCGAGGCCTA |
qRT-PCR |
335 bp |
|
[44] |
| DNMT1-Q-RE |
GTTGCAGTCCTCTGTGAACACTGTGG |
qRT-PCR |
|
|
|
| GAPDH-Q-FW |
CGACCACTTTGTCAAGCTCA |
qRT-PCR |
205 bp |
|
[45] |
| GAPDH-Q-RE | AGGGGTCTACATGGCAACTG | qRT-PCR |
COBRA, combined bisulfite restriction analysis: PSQ, pyrosequencing: qRT-PCR, quantitative real-time reverse-transcriptase polymerase chain reaction; Ref, reference.