Table 1.
Primers used in multi-locus sequence typing of Fusarium isolates collected from patients and the environment
| Locus | Gene product (size in bp) | Primer | Sequence 5’-3’† | PCR | Sequencing |
|---|---|---|---|---|---|
|
EF-1α |
Translation elongation factor 1 alpha (716) |
EF1 |
ATGGGTAAGGARGACAAGAC |
x |
|
| EF2 |
GGARGTACCAGTSATCATG |
x |
|
||
| EF3 |
GTAAGGAGGASAAGACTCACC |
|
x |
||
| EF22T |
AGGAACCCTTACCGAGCTC |
|
x |
||
|
rRNA |
Nuclear ribosomal rRNA (986) |
ITS5 |
GGAAGTAAAAGTCGTAACAAGG |
x |
x |
| ITS2 |
GCTGCGTTCTTCATCGATGC |
x |
x |
||
| ITS3 |
GCATCGATGAAGAACGCAGC |
x |
x |
||
| ITS4 |
TCCTCCGCTTATTGATATGC |
x |
x |
||
| NL4 |
GGTCCGTGTTTCAAGACGG |
x |
x |
||
|
RPB2 |
RNA Polymerase beta subunit (1738) |
5f2 |
GGGGWGAYCAGAAGAAGGC |
x |
|
| 7cr |
CCCATRGCTTGYTTRCCCAT |
x |
|
||
| 7cf |
ATGGGYAARCAAGCYATGGG |
x |
|
||
| 11ar |
GCRTGGATCTTRTCRTCSACC |
x |
|
||
| F2F |
AAAGCTCGCCAAGCCCCGTC |
|
x |
||
| F1R |
GTCCTCTGGTGGCTCCGCCT |
|
x |
||
| 40-2f |
CAAAAACCTCTGGCGACAAC |
|
x |
||
| 40-r | AGCTTGCGTCCAGTATGACC | x |
† Letter designations for degenerate bases: R, A or G; S, C or G; W, A or T; Y, C or T.