Table 2.

Primers for anaerobic hydrocarbon degradation genes detection

Primer Set	Forward (F) and Reverse (R) Oligonucleotide Primer Sequences	Expected amplicon size (bp)	Reference
SP9/ASP1 (bamA)	F: 5`-CAG TAC AAY TCC TAC ACV ACB G-3`	~300	[20]
	R: 5`-C MAT GCC GAT YTC CTG RC-3`
assA2F/R (assA)	F: 5’-YAT GWA CTG GCA CGG MCA-3’	440	Aitken et al., unpublished observations
	R: 5’-GCR TTT TCM ACC CAK GTA-3’
7772 F/8546R (bssA)	F: 5’-GAC ATG ACC GAC GCS ATY CT-3’	~794	[22]
	R: 5’-TCG TCG TCR TTG CCC CAY TT-3’

Oligonucleotide primers used in PCR reactions for anaerobic hydrocarbon degradation detection.