Figure 3. Volume measurement is robust to varying interval sizes.

WormSizer was used to segment 10 images of different nematodes (denoted by individual lines in the figure). The interval size (x-axis) was then varied and the resulting calculated volume reported. The average length of the worms was 396 pixels, so an interval size of 40 is approximately 10% of a worm's length. The mean CV of volume across worms across interval sizes was 2.3%.