Table 1. Repeated measurements of individual worms.
| 0 hours (L1 arrest) | Mean Volume picoliters (CV) | Mean Cylinder Volume picoliters (CV) | Mean Length µm (CV) | Mean Width µm (CV) |
| 1 | 48.0 (10%) | 65.2 (24%) | 201 (2.3%) | 20.2 (13%) |
| 2 | 41.4 (10%) | 54.9 (10%) | 198 (1.6%) | 18.8 (5.8%) |
| 3 | 47.5 (22%) | 65.8 (19%) | 201 (4.4%) | 20.3 (7.8%) |
| 4 | 50.2 (13%) | 58.5 (10%) | 200 (1.9%) | 19.3 (4.5%) |
| 5 | 61.3 (14%) | 85.8 (19%) | 251 (1.7%) | 20.8 (10%) |
| 6 | 42.7 (13%) | 69.0 (18%) | 202 (1.7%) | 20.8 (9.1%) |
| 7 | 48.3 (6.9%) | 66.4 (25%) | 200 (1.8%) | 20.4 (11%) |
| 8 | 40.1 (11%) | 54.2 (29%) | 191 (2.3%) | 18.8 (14%) |
| 9 | 53.3 (2.6%) | 76.4 (15%) | 201 (2.4%) | 21.9 (8.0%) |
| Mean | 48.1 (11%) | 66.3 (19%) | 205 (2.2%) | 20.2 (9.2%) |
| Sample CV | 15% | 21% | 6.7% | 11% |
| 48 hours | ||||
| 1 | 1.59E+03 (5.4%) | 2.16E+03 (12%) | 719 (3.7%) | 61.8 (5.9%) |
| 2 | 1.67E+03 (9.4%) | 2.22E+03 (13%) | 737 (2.2%) | 61.9 (5.6%) |
| 3 | 1.18E+03 (4.5%) | 1.64E+03 (6.5%) | 648 (3.1%) | 56.7 (2.4%) |
| 4 | 1.82E+03 (8.8%) | 2.48E+03 (10%) | 770 (1.6%) | 64.0 (4.3%) |
| 5 | 1.64E+03 (10%) | 2.28E+03 (16%) | 730 (5.7%) | 63.0 (7.3%) |
| 6 | 1.26E+03 (4.4%) | 1.74E+03 (8.0%) | 702 (2.5%) | 56.1 (4.8%) |
| 7 | 1.49E+03 (13%) | 2.19E+03 (13%) | 720 (7.3%) | 62.1 (4.1%) |
| 8 | 1.52E+03 (9.7%) | 2.16E+03 (14%) | 712 (5.3%) | 62.0 (6.6%) |
| 9 | 1.31E+03 (5.9%) | 1.92E+03 (5.0%) | 748 (2.5%) | 57.1 (2.0%) |
| 10 | 1.72E+03 (6.7%) | 2.33E+03 (11%) | 761 (3.5%) | 62.4 (4.4%) |
| 11 | 1.39E+03 (20%) | 1.84E+03 (17%) | 729 (5.3%) | 56.5 (6.8%) |
| Mean | 1.51E+03 (8.9%) | 2.09E+03 (11%) | 725 (3.9%) | 60.3 (4.9%) |
| Sample CV | 21% | 23% | 8.7% | 8.8% |
Worms at 0 hours and 48 hours (9 and 11 worms, respectively) were individually plated, imaged, and measured using WormSizer. Worms were measured in rounds (5 rounds total per time point), and the microscope's zoom, focus, and illumination were reset and calibrated according to protocol between rounds. Mean values and CVs are recorded per worm across all five rounds. The volume measurement is as computed by WormSizer; the cylinder volume calculation uses the worm's length and its width at the middle of its skeleton. The reported width is the width at the middle of the worm. The mean values of measurements and CVs are reported across worms. The sample CV is the mean CV across trials from the wild-type control in the main dataset.