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. 2013 Feb 22;8(2):e57601. doi: 10.1371/journal.pone.0057601

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© 2013 Ku et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lysates were layered onto 10–50% sucrose gradients for ultracentrifugation. Ten fractions were taken from top to bottom. (A) SDS-PAGE analysis. The ten fractions were separated by SDS-PAGE, and subsequently stained with Coomassie Blue dye. M, protein marker. EV, purified whole virion EV71 standard from Hualan Inc. (B) Western blotting with anti-VP0 polyclonal antibody. (C) Western blotting with anti-VP1 polyclonal antibody. (D) Western blotting with anti-VP3 polyclonal antibody.