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. 2013 Feb 22;8(2):e57601. doi: 10.1371/journal.pone.0057601

Figure 5. Antibody responses following VLP immunization.

Figure 5

Groups of five mice were injected i.p. at weeks 0, 2 and 5, with alum-absorbed antigens: EV71-VLP equivalent to 5 µg VP0, or the control lysate similarly prepared from uninfected Sf9 cells. The immunized mice were sacrificed at week 7 (2 weeks after the last immunization), and the serum, bone marrow and spleen samples were collected and assayed. Results are representative of two independent experiments. (A) EV71-specific serum IgG titers of the Sf9 and the VLP groups. Each symbol represents a mouse, and the line indicates the geometric mean value of the group. (B) ELISPOT analysis of EV71-specific antibody-secreting cells in mouse bone marrow. Samples from naïve mice were used as the control. Results of triplicate wells are shown. (C) ELISPOT analysis of EV71-specific antibody-secreting cells in mouse spleens. Samples from naïve mice were used as the control. Results of triplicate wells are shown.