Figure 8. Inhibitory effect of the antisera on EV71 attachment to cells.

Antisera at different dilutions (1:50, 1:500, and 1:5,000) were mixed with 400 µl of EV71 containing 9×10⁶ TCID₅₀, and incubated for 1 hour at 37 °C. The mixtures were added to (A) RD or (B) Vero cells for incubation for 1 hour at 4 °C to allow virus attachment to the cells. After incubation, the cells were washed with cold PBS three times, collected, and subjected to Western blotting with a polyclonal antibody against EV71 VP1. For loading control, an antibody against actin was also used in Western blotting.