Table 2. Primers and results of real-time qPCRs.
Gene | Primer | Product size (bp) | Fold change |
AASS | F: AGCGCTACATAAGTCGTT | 197 | 1.86 |
R: GTCACATATTGCCACGAGTTT | |||
CYP2J2 | F: AAAGAAGCCCTTATCCAC | 187 | 30.53 |
R: GAATGCGTTCCTCTAAGCTCT | |||
CYP4A11 | F: CCTGGACCAGATGCCCTACAC | 107 | 111.92 |
R: CAGGGAAGGTGACGGGAGT | |||
PLA2G2A | F: CATGATCTTTGGCCTACTGC | 108 | 6.57 |
R: CAGCCGTAGAAGCCATAACTG | |||
PLA2G5 | F: ACATTCGCACACAGTCCTAC | 170 | 24.23 |
R: GAGGATGTTGGGAAAGTATTG | |||
PTEN | F: CAGAAAGACTTGAAGGCGTAT | 124 | 52.53 |
R: TTGGCGGTGTCATAATGT | |||
RB1 | F: TCCTCGAAGCCCTTACAAGTT | 176 | 6.38 |
R: TCTCAGAAGTCCCGAATGAT |
Note: These primers were designed by using Oligo6. Fold changes were computed by comparing between HGG and LGG.