Table 1. Plasma membrane and protrusion stiffness and viscosity.

Plasma membrane cholesterol content (pmol/µg protein)	Membrane tether stiffness (pN/µm) [13]	Membrane tether effective viscosity (pN.s/µm) [13]	Protrusion stiffness (k 0) (pN/µm)	Protrusion stiffness (k 1) (pN/µm)	Protrusion viscosity (η 0)(pN.s/µm)	Protrusion stiffness (k 0) + Lat-A (pN/µm)	Protrusion stiffness (k 1) + Lat-A (pN/µm)	Protrusion viscosity (η 0)+ Lat-A (pN.s/µm)
5.68	3.24±0.1	Not determined¶	607±130	59±15	152±40	206±67	27±12	74±23
7.54	1.2±0.19	2.6±1	349±121	32±15	132±36	133±91	14±9	62±21
17.3	0.28±0.01	3.6±0.73	135±92	19±13	71±30	88±52	12±8	31±24

Plasma membrane cholesterol concentration in control cells is 7.54 pmol/µg protein. Lat-A represents treatment of the cells with Latrunculin-A in order to disrupt F-actin polymerization.

^¶Membrane tether effective viscosity was not determined at 5.68 pmol/µg protein cholesterol concentration, but its value under cholesterol depleted conditions at 6.6±0.3 pmol/µg protein was ≈2.1±0.4 pN.s/µm [13].