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. 2013 Feb 22;8(2):e56582. doi: 10.1371/journal.pone.0056582

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© 2013 Voulgaridou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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SDS-PAGE analysis at various stages of purification of recombinant his-fused ALDH3A1 using Ni-affinity chromatography (Coomassie blue staining). T₀: Total cell extract form bacterial culture co-expressing pG-KJE8 along with ALDH3A1 prior to IPTG induction, T₆: Total cell extract 6 hours after IPTG induction, CE: Crude extract of lysed cells 6 hours after IPTG induction, IM: Insoluble matter of lysed cells 6 hours after IPTG induction, In: Input of the column, Fl: flowthrough part, W: wash part, Elution fractions: purified recombinant protein eluted from Ni-NTA column. WB: western immunoblotting of purified recombinant ALDH3A1/6xHis. The arrow indicates the position of the recombinant his-tagged ALDH3A1 at approximately 51 kDa.