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| Purification Steps | Total Protein (mg) | Yield (%) | Specific Activityb (mU/mg protein) | Purification (fold) |
| Crude supernatanta | 36 | 100 | 200 | 1 |
| Ni-NTA column | 2.43 | 6.75 | 1150 | 5.75 |
The starting material was 250 ml of crude E. coli supernatant.
One milliunit (mU) of activity was defined as the amount of activity that oxidized of 1 nmol of NADPH/min at 25°C. Representative results of three different isolation procedures.
