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. 2013 Feb 22;8(2):e56634. doi: 10.1371/journal.pone.0056634

Figure 4. Overexpression of miR-195 inhibited cell viability and cell cycle progression and promoted cell apoptosis.

Figure 4

(A), Inhibition of cell viability by overexpression of miR-195. SCC-15 and CAL27 cells were transfected with pcDNA3.0, a negative control (NC) or with pcDNA3.0-miR-195 (miR-195), as indicated. Cell viability was measured using CCK-8 assays. The data were presented as means ± SD (n = 5) (* P<0.05, ** P<0.01). (B), Inhibition of cell cycle progression by overexpression of miR-195. SCC-15 and CAL27 cells were transfected as in (A). Cells were stained with propidium iodide (PI) at 48 h post-transfection and analyzed with FACS (* P<0.05, ** P<0.01). (C), Promotion of apoptosis by overexpression of miR-195. SCC-15 or CAL27 cells were transfected for 48 h as in (A) and apoptotic cells were monitored with FACS after Annexin V and PI staining (*** P<0.001).