Figure 4. Confocal and flow cytometric analysis of RWPE-1 cells.

(A) Confocal immunofluorescence images of MUC1 (red) and sLe^a (green) on RWPE-1 cells treated with PBS (control) or SAHA (5 µM) for 72 h. SLe^a induced by SAHA is colocalized with MUC1. (B) SLe^a mean fluorescence intensity in RWPE-1 cells treated with PBS or SAHA as measured by flow cytometry analysis. (p < 0.05) (C) % of sLe^a-positive cells in PBS- or SAHA-treated cells. The data expressed as mean ± SEM were obtained from three independent experiments. (p < 0.05) Bar  =  20 µm.