Figure 2. Cleavage of CSP mRNA sequence (substrate csp38) by human RNase P in the presence of different EGSs. No EGS was added to the reaction mixture in lane 1. EGS CSP-SER (10 nM) (lane 3), CSP-C321 (2 nM) (lane 2), CSP-C321-C (10 nM) (lane 4), and TK112 (10 nM) (lane 5) were incubated with [³²P]-labeled CSP RNA substrate (20 nM) and human RNase P (2 units) at 37°C in a volume of 10 μl for 45 min in buffer A (50 mM Tris, pH 7.4, 100 mM NH₄Cl, and 10 mM MgCl₂). Experimental details can be found in Materials and Methods.