Figure 5. Expression of human actin and HCMV proteins as assayed by western blot analysis. Protein samples were isolated from the parental U373MG cells or EGS-expressing cells that were either mock-infected (lanes 1 and 6) or infected with HCMV (MOI = 0.5–1) (lanes 2–5 and 6–10) for 48 h, separated in either SDS-polyacrylamide gels, transferred to membranes, and reacted with antibodies against human actin (A) and HCMV CSP (B). The double bands in (B) represent the full length and processed CSP protein.⁷