Figure 5. The co-cultured BMDCs and CD8+ T cells (A) in a range of different ratio under a light microscope (200 ×). The purified CD8+T cells were incubated with BMDCs (BMDCs/CD8+ T cells in a ratio of 1:5, 1:10, 1:20 and 1:40) for 5d (B). The BMDCs/CD8+T cells at ratio 1:10 showed the best effect on driving CD8+ T cells proliferation. Up-regulation of CD28, FasL and Prf on CD8+ T cells after treatment with RG and/or MENK BMDCs for 5d (C). The expression of markers was analyzed by FCM and was displayed respectively by the single parameter diagram. The values shown in the profiles were the gated %. Results represent the mean ±SD of three independent samples.