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. 2013 Feb 22;8(2):e56892. doi: 10.1371/journal.pone.0056892

Figure 3. Integration activities of RSV IN (1–270) mutants and 2-domain IN constructs.

Figure 3

RSV IN (1–270) constructs with the mutations as indicated at the top (lanes 1 to 12), and the two-domain IN construct lacking either the CTD (lanes 13 and 14) or the NTD (lanes 15 to 18) were analyzed at protein concentrations of 8 nM and 12 nM, in an assay condition containing 100 mM NaCl. The control reaction was without IN marked C (lane 19). The strand transfer products and the 3.6 kb GU3 donor are indicated on the left.