Figure 5. Inhibitory effects of no.8016 and four ARBs on cell proliferation.

(A-D) Inhibitory effects of compound no.8016 (A) and four ARBs (B) on TPA-induced cell proliferation in keratinocytes with a cell proliferation assay. 2×10⁴ cells were treated in conditioned media with or without the no.8016 or four ARBs during TPA stimulation, and absorbance at 450 nm was determined every 12 h till 60 h. (A, C) Inhibition of cell proliferation with no.8016 and telmisartan was verified following a wash at 12 h. (D) The cells were also observed with microscopy. **P<0.05 for the inhibitory effects during 12 candidates and four ARBs treatment vs. TPA treatment.