Figure 3. Mixed species biofilms inhibit epithelialization in porcine partial thickness wound model on day 2. A.

Simultaneous infection with USA300 and P. aeruginosa 09-010 (PA) significantly inhibited epithelialization (* p≤0.05) when compared to wounds infected with single species or control (Ctrl) uninfected wounds (** p≤0.001). USA300 delayed epithelialization when compared to uninfected wounds (Ctrl) (** p≤0.001). B. Representative wounds stained with H&E are shown. White arrows indicate wound edges after initial wounding while black arrowheads point at the epithelialized edges of the migrating fronts. CR = crust.