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. Author manuscript; available in PMC: 2014 Feb 22.
Published in final edited form as: J Nat Prod. 2013 Jan 17;76(2):243–249. doi: 10.1021/np3007414

Figure 4. Effects of compounds 1–6 on recombinant nAChRs.

Figure 4

An inhibition assay for each compound was performed using HEK tsA201 cells stably expressing hα3β4 and hα4β2 nAChRs. Cells were loaded with Calcium 5 NW dye and stimulated with 1 μM epibatidine in the presence of a single concentration of each test compound (10 μM), as described in the Experimental Section. Results are expressed as the percentage of the control, epibatidine-stimulated peak fluorescence level. Values represent the means ± SD of three to five experiments performed in triplicate. Percentage inhibitions achieved by compounds are shown in Table 2.