Fig. 4.

IL-1β induced higher gene expressions of MMPs in injured ACL than those in MCL fibroblasts. a Quantitative real-time PCR showed higher gene expressions of MMP-1, -2 and -12 in injured ACL than those in MCL fibroblasts. The data were recorded after 0 (control), 2, 6, 12 and 24 h following 5 ng/ml IL-1β treatments. The data for each sample were normalised to GAPDH mRNA. Data (means ± SD, n = 4) were represented as the fold change in expression compared to control. *p < 0.05. b The mRNA of MMP-3 in ACL and MCL cells were too low to detect using quantitative real-time PCR by the △Ct method loading 1 μl cDNA coordinate with other MMPs. Through GAPDH using semi-quantitative PCR with 38 cycles, increased gene expressions reached value peaks at 6 h after 5 ng/ml IL-1β in injured ACL and MCL. Notably, the ratio of MMP-3 mRNA (injured ACL to MCL) was high. The data were the mean of four different experiments (n = 4). *sSgnificant difference with respect to control (p < 0.05)