Table 3.
Effect of serum heat-inactivation on M. tuberculosis association with monocytes from participants with and without diabetes
| Diabetes classification | PID |
M. tuberculosis association (% monocytes infected)
|
Fraction of association attributed to heat-labile factora | P values
|
|||
|---|---|---|---|---|---|---|---|
| Fresh serum | Heat-inactivated serum | Fresh serum | Heated serum | Association due to heat-labile factor | |||
| No DM | 5–133 | 31.2 | 6.9 | 78% | REF | REF | REF |
| No DM | 5–135 | 21.0 | 3.0 | 86% | |||
| No DM | 5–141 | 32.0 | 19.5 | 39% | |||
| No DM | 5–147 | 29.0 | 0.0 | 100% | |||
| No DM | 5–149 | 39.5 | 8.9 | 77% | |||
| No DM | 5–153 | 17.5 | 6.0 | 66% | |||
|
| |||||||
| Mean (SD) | 28.4(8) | 7.4(6.7) | 74.3%(20.6) | ||||
|
| |||||||
| DMb | 5–134 | 24.9 | 6.0 | 76% | 0.04 | 0.69 | 0.06 |
| DMb | 5–137 | 31.0 | 9.0 | 71% | |||
| DMb | 5–143 | 23.5 | 9.0 | 62% | |||
| DMb | 5–150 | 12.0 | 6.0 | 50% | |||
| DMp | 5–101 | 11.9 | 9.2 | 23% | |||
| DMp | 5–114 | 10.4 | 7.1 | 32% | |||
| DMp | 5–139 | 19.0 | 17.0 | 11% | |||
| DMp | 5–140 | 20.0 | 12.5 | 38% | |||
| DMp | 5–142 | 23.5 | 5.5 | 77% | |||
| DMp | 5–146 | 22.0 | 4.0 | 82% | |||
|
| |||||||
| Mean (SD) | 17.8(5.4) | 10.1(5) | 37.6%(33) | ||||
Mycobacteria was added at a 10:1 MOI in 20% fresh or heated autologous serum for 2h (see Methods);
a
Fraction of association=1-(%monocyte association in Heat-inactivated serum/%monocyte association in fresh serum);
DMb, diabetes with borderline glucose control; DMp, diabetes with poor glucose control; PID, participant identification; M. tuberculosis, M. tuberculosis.