Fig. 6.

Triple immunofluorescent labeling for the astroglial marker GFAP (a–h; green) or the microglial marker HLA-DR (i–t; green), the nuclear marker Hoechst 33342 (blue), and angiotensinogen/angiotensin (Ang; red) in monkey and human substantia nigra compacta. In astrocytes, labeling is mainly located at the cytoplasmic level. In microglial cells of normal substantia nigra, labeling for angiotensinogen/angiotensin was weak and particularly located at the cell surface (arrows). Ang angiotensinogen/angiotensin, GFAP glial fibrillary acidic protein, HLA-DR human leukocyte antigen DR, SC Santa Cruz Biotechnology, RD R&D Systems. Scale bar 20 µm