Table 1.
Primary antibodies
| Antibody specificity | Host species | Type (clone) | Isotype | Dilution | Manufacturer (cat. no.) |
|---|---|---|---|---|---|
| Angiotensin II type 1 receptor | Rabbit | Polyclonal | IgG | IP: 1:100 | Santa cruz biotechnology (sc-579) |
| IF: 1:50 | |||||
| Angiotensin II type 2 receptor | Rabbit | Polyclonal | IgG | IP: 1:100 | Santa cruz biotechnology (sc-9040) |
| IF: 1:50 | |||||
| Angiotensinogen | Goat | Polyclonal | IgG | IP: 1:200 | R&D systems (AF3156) |
| IF: 1:100 | |||||
| Angiotensinogen, angiotensin I, II and III | Goat | Polyclonal | IgG | IP: 1:1,000 | Santa cruz biotechnology (sc-7419) |
| IF: 1:500 | |||||
| Glial fibrillary acidic protein | Mouse | Monoclonal (GA5) | IgG1 | IF: 1:500 | Millipore (MAB360) |
| HLA-DR | Mouse | Monoclonal (169-1B5) | IgG2b | IF: 1:50 | MP biomedicals (68549) |
| HLA-DR | Mouse | Monoclonal (LN-3) | IgG2b | IF: 1:50 | Novocastra (NCL-LN3) |
| Renin/prorenin receptor | Rabbit | Polyclonal | IgG | IP: 1:100 | Abcam (ab40790) |
| IF: 1:50 | |||||
| Tyrosine hydroxylase | Mouse | Monoclonal (TH-16) | IgG | IF: 1:5,000 | Sigma (T2928) |
IP immunoperoxidase labeling method, IF immunofluorescence labeling method