Fig. 3.

RANKL-induced activation of NF-κB and MAPKs remains intact when BMMs are simultaneously exposed to IL-4 and RANKL. BMMs were treated with IL-4 (4 ng/ml) and RANKL (100 ng/ml) as indicated. Cells were lysed and analyzed by Western blotting using antibodies against phospho-IκB (p-IκB), phospho-ERK (p-ERK), phospho-JNK (p-JNK), and phospho-p38 (p-p38). The same volume of lysates were run and then probed with antibodies against IκB, ERK, JNK, and p38 as loading controls. [Color figure can be seen in the online version of this article, available at http://wileyonlinelibrary.com/journal/jcb]