Figure 4. Dicer is not required for the formation of BAT in vivo.
(A) H&E and ORO staining reveal that lipogenesis is not compromised in the intrascapular BAT of Dicer-ablated (mutant) mice. Scale bar represents 80μm (B) Quantitative PCR results of adipogenic and thermogenic-related transcripts present in BAT of 10-day old Dicer-wildtypemice and Dicer-mutant mice. Analysis of Dicer-wildtype and Dicer-mutant spleen was performed as control. The average value for each wildtype transcript was set at 1. Bars represent SD values obtained from eight sibling pairs wherein each pair consists of one mutant and one wildtype mouse.