Fig. 1.
Representative plots showing O2 binding equilibrium curves (OECs) for the 60 μM (in heme) solutions of the Hb–Hp complex (– – – –) and for uncomplexed Hb (– –) measured 10 mM potassium phosphate/0.1 M NaCl, pH 7.4 at 37 °C. The average P50 and n50 values derived from three separate experiments are reported in Table 1. Insert for this figure shows the respective Hill plots for each curve.